Southern pine beetle-specific RNA interference exhibits no effect on model nontarget insects
نویسندگان
چکیده
Abstract The efficacy and high specificity of the RNA interference pathway has prompted its exploration as a potential molecular management tool for many insect pests, including destructive southern pine beetle, Dendroctonus frontalis Zimmermann, in which gene knockdown mortality via double-stranded RNAs (dsRNAs) have already been demonstrated laboratory. nucleotide sequence dsRNAs requires an exact match at least 16 nucleotides with targeted messenger to trigger that gene. This allows vital genes target pest be silenced induced while reducing probability adverse effects nontarget organisms. However, prior utilization forest ecosystems, demonstration through laboratory bioassays evaluating on model insects is required proper risk assessment analyses. Consequently, we evaluated three SPB-specific lethal effects, sublethal (larval growth rate, adult emergence or fecundity), relative expression representing key functional guilds, predator, herbivore, pollinator. had no effect survival our insects. Additionally, were found analyses corroborated bioinformatic finding knockdown. Our findings support RNAi technology provide development deployment protection conifer forests against SPB minimal concerns.
منابع مشابه
The Southern Pine Beetle and Pine Forest Ecosystems
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ژورنال
عنوان ژورنال: Journal of Pest Science
سال: 2022
ISSN: ['1612-4758', '1612-4766']
DOI: https://doi.org/10.1007/s10340-021-01473-1